ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (5): 821-826.doi: 10.11843/j.issn.0366-6964.2014.05.021

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Study of the Dynamic Distribution of uNK Cells and VEGF in the Pregnant Goat

DING Pei-yang,LIU Yan-li,CAO Yang-po,LI Xiao,JIN Ya-ping,WANG Ai-hua,QI Xue-feng*   

  1. (College of Veterinary Medicine,Northwest A &F University ,Yangling 712100, China)
  • Received:2013-06-26 Online:2014-05-23 Published:2014-05-23

Abstract:

The aim of this study was to investigate the relationship between the dynamic distribution of uNK cells and VEGF in uterus of the pregnant goat.The distribution and content of uNK cells and VEGF in the pregnant uterus were detected by using immunohistochemical technology,and the transcription level of VEGF mRNA was examined by RT-PCR assay.We found that during early pregnancy,uNK cells and VEGF were concentrated in deciduas; during mid trimester of pregnancy,uNK cells and VEGF were concentrated on stroma region,gland and vessel in muscle.However,the positive staining for uNK cells and VEGF was significantly decreased in late trimester of pregnancy.The results of RT-PCR showed that in early and mid trimester of pregnancy,the transcription of VEGF mRNA had no significant deviation,but significantly higher than that of VEGF mRNA in late trimester of pregnancy (P<0.05).The results suggest that the distribution and content of uNK cells and VEGF are relevant to some extent in different pregnant period.

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